Protocols

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Download and make sure to follow the guidelines below before proceeding:

• EpiGe_Genotyping_Protocol.pdf
• EpiGe_Genotyping_Template.pdf
• EpiGe_QuantStudio_Configuration.pdf

Download and make sure to use the corresponding template for your equipment:

• EpiGe_TEMPLATE_7500.edt
• EpiGe_TEMPLATE_Q6Flex.edt
• EpiGe_TEMPLATE_QS1.edt
• EpiGe_TEMPLATE_QS3.edt
• EpiGe_TEMPLATE_QS5.edt

Materials

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The following table shows the name of the primers, the associated CpG in the methylation arrays and the link to purchase the primer:

#	EpiGe_primer_ID	Illumina CpG ID	IDT_Primer_ID	Link to purchase
1	G1_1884	cg18849583	CD.GT.WFVP0601.1
2	G3_0126	cg01268345	CD.GT.PVWC1084.1
3	S1_1033	cg10333416	CD.GT.SPDR8897.10
4	S3_1292	cg12925355	CD.GT.GYJV9231.1
5	W1_2554	cg25542041	CD.GT.JBHB3172.1
6	W3_0222	cg02227036	CD.GT.JYVK6101.1

Supplementary materials

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The following file is an example of a thermal cycler generated file. This file contains amplification data from a medulloblastoma sample using the Applied Biosystems QS5 thermocycler. It can be used to test the webserver without the need for your own data.

• EpiGe_sample_test.txt

The following file is an example of a report generated by the webserver. Users can download their own analysis reports, such as this one, on their results pages. For a better understanding of the results the user can consult the tutorial or read the article published in iScience.

• EpiGe_report_example.pdf